Detecting hybridization in African schistosome species: does egg morphology complement molecular species identification?

作者: NELE A. M. BOON , WOUTER FANNES , SARA ROMBOUTS , KATJA POLMAN , FILIP A. M. VOLCKAERT

DOI: 10.1017/S0031182017000087

关键词: Hybrid zoneSchistosoma haematobiumBiologyHybridContext (language use)EcologyMorphology (linguistics)VirulenceHuman parasiteParasite hostingZoology

摘要: Hybrid parasites may have an increased transmission potential and higher virulence compared to their parental species. Consequently, hybrid detection is critical for disease control. Previous crossing experiments showed that schistosome eggs distinct morphotypes. We therefore the performance of egg morphology with molecular markers regard detecting hybridization in schistosomes. studied 303 terminal-spined eggs, originating from 19 individuals inhabiting a zone natural crosses between human parasite Schistosoma haematobium livestock bovis Senegal. The sizes high variability ranged 92·4 176·4 µm length 35·7 93·0 width. No morphotypes were found all resembled, varying extent, typical S. type. However, analyses on same clearly presence two partial mitochondrial cox1 profiles, namely haematobium, only single nuclear ITS rDNA profile (S. haematobium). Therefore, these particular crosses, appears not good indicator ancestry. conclude by discussing strengths limitations methods detect hybrids context high-throughput screening field samples.

参考文章(46)
Lynn Meurs, Eric Brienen, Moustapha Mbow, Elizabeth A. Ochola, Souleymane Mboup, Diana M. S. Karanja, W. Evan Secor, Katja Polman, Lisette van Lieshout, Is PCR the Next Reference Standard for the Diagnosis of Schistosoma in Stool? A Comparison with Microscopy in Senegal and Kenya. PLOS Neglected Tropical Diseases. ,vol. 9, pp. 9- ,(2015) , 10.1371/JOURNAL.PNTD.0003959
Chaves A, Katz N, Pellegrino J, A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Revista Do Instituto De Medicina Tropical De Sao Paulo. ,vol. 14, pp. 397- 400 ,(1972)
Jérôme Boissier, Hélène Moné, Guillaume Mitta, M Dolores Bargues, David Molyneux, Santiago Mas-Coma, Schistosomiasis reaches Europe. Lancet Infectious Diseases. ,vol. 15, pp. 757- 758 ,(2015) , 10.1016/S1473-3099(15)00084-5
Hélène Moné, Martha C. Holtfreter, Jean-François Allienne, Rodrigue Mintsa-Nguéma, Moudachirou Ibikounlé, Jérôme Boissier, Antoine Berry, Guillaume Mitta, Joachim Richter, Gabriel Mouahid, Introgressive hybridizations of Schistosoma haematobium by Schistosoma bovis at the origin of the first case report of schistosomiasis in Corsica (France, Europe) Parasitology Research. ,vol. 114, pp. 4127- 4133 ,(2015) , 10.1007/S00436-015-4643-4
Kayla C. King, Rike B. Stelkens, Joanne P. Webster, Deborah F. Smith, Michael A. Brockhurst, Hybridization in Parasites: Consequences for Adaptive Evolution, Pathogenesis, and Public Health in a Changing World. PLOS Pathogens. ,vol. 11, ,(2015) , 10.1371/JOURNAL.PPAT.1005098
M. G. Taylor, Hybridisation experiments on five species of African schistosomes. Journal of Helminthology. ,vol. 44, pp. 253- 314 ,(1970) , 10.1017/S0022149X00021969
Douglas Bates, Martin Mächler, Ben Bolker, Steve Walker, Fitting Linear Mixed-Effects Models Using lme4 Journal of Statistical Software. ,vol. 67, pp. 1- 48 ,(2015) , 10.18637/JSS.V067.I01
Paul J. Brindley, Peter J. Hotez, Break Out: urogenital schistosomiasis and Schistosoma haematobium infection in the post-genomic era. PLOS Neglected Tropical Diseases. ,vol. 7, ,(2013) , 10.1371/JOURNAL.PNTD.0001961
A D Twyford, R A Ennos, Next-generation hybridization and introgression Heredity. ,vol. 108, pp. 179- 189 ,(2012) , 10.1038/HDY.2011.68