Increase of mitochondria surrounding spindle causes mouse oocytes arrested at metaphase I stage.
作者： Xing-Hua Wang , Shen Yin , Xiang-Hong Ou , Shi-Ming Luo
DOI: 10.1016/J.BBRC.2020.05.037
关键词: Chemistry 、 Germinal vesicle 、 MFN2 、 Membrane potential 、 Bacterial outer membrane 、 Endoplasmic reticulum 、 Oocyte 、 Chromosome segregation 、 Cell biology 、 Mitochondrion
摘要: During oocyte meiosis, mitochondria usually surround spindle to meet the energy demand of migration and chromosome segregation. Therefore, mitochondrion surrounding is widely accepted as an important indicator demonstrate mitochondrial function in studies. However, role quality not exactly addressed. Mitofusin-2 (MFN2) a outer membrane GTPase that mediates clustering fusion. Here, we increased by overexpression MFN2 mouse oocytes. Results indicate increase has little effect on germinal vesicle breakdown (GVBD), migration, potential (MMP), reactive oxygen species (ROS) production Endoplasmic reticulum (ER) distribution, while blocks segregation, destroys spindle, finally causes most oocytes arrest at metaphase I stage. Collectively, our results precisely regulated during maturation, too much it may cause abnormal meiosis. although typical biological event utilizing should be careful.
sci-hub.st 参考文章(24)
Bernard Maro, Marie-Hélène Verlhac, Polar body formation: new rules for asymmetric divisions. Nature Cell Biology. ,vol. 4, ,(2002) , 10.1038/NCB1202-E281
Chin-San Liu, Jui-Chih Chang, Shou-Jen Kuo, Ko-Hung Liu, Ta-Tsung Lin, Wen-Ling Cheng, Sheng-Fei Chuang, Delivering healthy mitochondria for the therapy of mitochondrial diseases and beyond. The International Journal of Biochemistry & Cell Biology. ,vol. 53, pp. 141- 146 ,(2014) , 10.1016/J.BIOCEL.2014.05.009
D. K. Slonim, K. Koide, K. L. Johnson, U. Tantravahi, J. M. Cowan, Z. Jarrah, D. W. Bianchi, Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses Proceedings of the National Academy of Sciences of the United States of America. ,vol. 106, pp. 9425- 9429 ,(2009) , 10.1073/PNAS.0903909106
Yuansong Yu, Remi Dumollard, Andreas Rossbach, F. Anthony Lai, Karl Swann, Redistribution of mitochondria leads to bursts of ATP production during spontaneous mouse oocyte maturation Journal of Cellular Physiology. ,vol. 224, pp. 672- 680 ,(2010) , 10.1002/JCP.22171
Hsiuchen Chen, Scott A. Detmer, Andrew J. Ewald, Erik E. Griffin, Scott E. Fraser, David C. Chan, Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development Journal of Cell Biology. ,vol. 160, pp. 189- 200 ,(2003) , 10.1083/JCB.200211046
Dean Clift, Melina Schuh, Restarting life: fertilization and the transition from meiosis to mitosis Nature Reviews Molecular Cell Biology. ,vol. 14, pp. 549- 562 ,(2013) , 10.1038/NRM3643
A.H. Sathananthan, A.O. Trounson, Mitochondrial morphology during preimplantational human embryogenesis. Human Reproduction. ,vol. 15, pp. 148- 159 ,(2000) , 10.1093/HUMREP/15.SUPPL_2.148
B. D. Bavister, J. M. Squirrell, Mitochondrial distribution and function in oocytes and early embryos Human Reproduction. ,vol. 15, pp. 189- 198 ,(2000) , 10.1093/HUMREP/15.SUPPL_2.189
So I. Nagaoka, Terry J. Hassold, Patricia A. Hunt, Human aneuploidy: mechanisms and new insights into an age-old problem Nature Reviews Genetics. ,vol. 13, pp. 493- 504 ,(2012) , 10.1038/NRG3245
Anne Lombès, Frédéric Legros, Danielle Chateau, Manuel Rojo, Membrane topology and mitochondrial targeting of mitofusins, ubiquitous mammalian homologs of the transmembrane GTPase Fzo Journal of Cell Science. ,vol. 115, pp. 1663- 1674 ,(2002) , 10.1242/JCS.115.8.1663