Nuclear localization and DNA binding of the transforming gene product of avian myelocytomatosis virus

摘要: Oncornaviruses transform cells either directly through a virus-coded oncogene1 or, if they lack such gene, indirectly by promoter insertion2–4 into the cellular genome and activation of gene. Both transformation mechanisms ultimately result in abnormally high expression normal genes. Recently, activated gene certain lymphomas4 has been shown to be homologous oncogene an acute avian leukaemia virus, MC29. In MC29 is fused viral structural gag. The product this protein molecular weight 110,000 (110 K)5–7, designated p110gag–myc. We have characterized using monoclonal antibodies against p19, N-terminal portion gag–myc fusion or 110 K purified it 3,700-fold immune affinity column chromatography. Immunofluorescence studies cell fractionation MC29-transformed fibroblasts indicate that predominantly located nucleus. Moreover, binds double-stranded DNA. These properties may related role transformation.