Human peripheral blood CD34+ cells attenuate oleic acid–induced acute lung injury in rats

摘要: Abstract Background aims Adult stem cell–based therapy is a promising novel approach for treatment of acute lung injury (ALI). In this study, we evaluated the therapeutic effect isolated human peripheral blood CD34+ progenitor cells in an ALI rat model, induced by oleic acid (OA) injection. Methods Seventy-five adult female rats were used study. Group A, control without treatment, and group B, injected with phosphate-buffered saline, comprised 15 each; remaining 45 OA to induce further subdivided into 3 groups: C (ALI group, rats), D fibroblast rats) E cell rats). Results transplantation OA-induced improves arterial PaO 2 wet/dry ratio, reduces infiltration inflammatory decreases vascular permeability as determined reduced intra-alveolar interstitial patchy congestion hemorrhage well decreased edema. Additionally, inflammation expression pro-inflammatory mediators intercellular adhesion molecule 1 tumor necrosis factor -α was attenuated cell–treated at 6, 24 48 h post-OA challenge compared non-treated rats. Moreover, anti-inflammatory interleukin-10 up-regulated after administration cells. The important finding that TNF-α-induced protein 6 ( TSG-6 ) gene significantly treated Conclusions freshly blood–derived may be source improve ALI. properties are explained, least part, activation express .