SIRT6 Is Essential for Adipocyte Differentiation by Regulating Mitotic Clonal Expansion.
作者： Qiang Chen , Wenhui Hao , Cuiying Xiao , Ruihong Wang , Xiaoling Xu
DOI: 10.1016/J.CELREP.2017.03.006
关键词: Mitosis 、 Cell biology 、 Adipogenesis 、 Sirtuin 、 SIRT6 、 Biochemistry 、 Kinase activity 、 NAD+ kinase 、 Biology 、 Kinesin 、 Gene expression
摘要: Summary Preadipocytes initiate differentiation into adipocytes through a cascade of events. Mitotic clonal expansion, as one the earliest events, is essential for adipogenesis. However, underlying mechanisms that regulate mitotic expansion remain elusive. SIRT6 member evolutionarily conserved sirtuin family nicotinamide adenine dinucleotide (NAD)+-dependent protein deacetylases. Here, we show deficiency in preadipocytes blocks their Analysis gene expression during adipogenesis reveals KIF5C, which belongs to kinesin family, negatively regulated by SIRT6. Furthermore, KIF5C negative factor interacting with CK2α′, catalytic subunit CK2. This interaction CK2α′ nuclear translocation and CK2 kinase activity inhibits These findings reveal crucial role provide potential therapeutic targets obesity.
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