Temperature-Controlled High-Speed AFM: Real-Time Observation of Ripple Phase Transitions
作者： Hirohide Takahashi , Atsushi Miyagi , Lorena Redondo-Morata , Simon Scheuring
关键词: Hysteresis 、 Transition temperature 、 Phase transition 、 Chemical physics 、 Phase (matter) 、 Chemistry 、 Ripple 、 Condensation 、 Lipid bilayer 、 Metastability 、 Analytical chemistry
摘要: With nanometer lateral and Angstrom vertical resolution, atomic force microscopy (AFM) has contributed unique data improving the understanding of lipid bilayers. Lipid bilayers are found in several different temperature-dependent states, termed phases; main phases solid fluid phases. The transition temperature between is composition specific. Under certain conditions some adopt a so-called ripple phase, structure where phase domains alternate with constant periodicity. Because its narrow regime existence heterogeneity dynamics remain poorly understood. Here, control device to high-speed (HS-AFM) observe from reversibly real time developed integrated. Based on HS-AFM imaging, processes metastable could be reversibly, phenomenologically, quantitatively studied. results here show hysteresis fast cooling heating processes, while both melting condensation occur at 24.15 °C quasi-steady state situation. A second larger periodicity formed when buffer contains Ca2+. presented temperature-controlled new experimental system temperature-sensitive nanoscopic level.
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