Detection of c-ras gene mutation and expression of p21 protein in dysplasias and carcinomas complicating ulcerative colitis

摘要: Point mutations of c-ras genes and the expression p21 protein were analyzed in 13 patients with colorectal carcinoma complicating ulcerative colitis; 12 13, there dysplastic lesions close to carcinomas. studied by polymerase chain reaction dot blot hybridization 32p-labeled oligonucleotide probes. Findings indicated c-Ki-ras at condons 12, 61 c-Ha-ras c-N-ras 61. The was immunohistochemical staining using a monoclonal antibody. Only 1 showed point mutation, this being from G A transition second position codon c-Ki-ras. No c-Ki-ras, c-Ha-ras, or found other carcinomatous dysplasias. In 11 patients, increased observed. These results suggest that are rare dysplasias carcinomas colitis is not always correlated genes.