Enzymes Involved in the Biosynthesis of Arginine from Ornithine in Maritime Pine (Pinus pinaster Ait.).

摘要: The amino acids arginine and ornithine are the precursors of a wide range nitrogenous compounds in all living organisms. metabolic conversion into is catalyzed by sequential activities enzymes transcarbamylase (OTC), argininosuccinate synthetase (ASSY) lyase (ASL). Because their roles urea cycle, these have been purified extensively studied variety animal models. However, available information about molecular characteristics, kinetic regulatory properties relatively limited plants. In conifers, plays crucial role as main constituent N-rich storage proteins seeds serves source nitrogen for germinating embryo. this work, recombinant PpOTC, PpASSY PpASL from maritime pine (Pinus pinaster Ait.) were produced Escherichia coli to enable study kinetics properties. results reported here provide basis regulation metabolism at enzymatic level, suggesting that reaction OTC target homeostasis pools can be either used biosynthesis plastids or other cytosol.