Qualitative polymerase chain reaction methods for detecting major food allergens (peanut, soybean, and wheat) by using internal transcribed spacer region.

摘要: Allergen detection methods for peanut, soybean, and wheat were developed by designing PCR primer pairs specific amplification of a fragment the internal transcribed spacer (ITS) region reported Arachis spp. Glycine Triticum Aegilops wheat. The target species included not only cultivated, but also wild ancestor species, which thought to be potentially allergenic. ability resultant detect was verified using genomic DNA extracted from A. hypogaea peanut G. max soybean; T. aestivum, turgidum, durum, aestivum-rye amphidiploid, monococcum, timopheevi, Ae. speltoides, squarrosa LODs 50-500 fg DNA, comparable those most sensitive previously reported. results present work, as well our previous work on buckwheat kiwifruit, prove that ITS region, its high copy number interspecific diversity, is particularly useful allergen methods.