Characterization of the interaction between mercaptoethylamine capped CdTe quantum dots with human serum albumin and its analytical application
作者： Youqiu He , Pengfei Yin , Huiping Gong , Juanjuan Peng , Shaopu Liu
DOI: 10.1016/J.SNB.2011.03.019
关键词: Fluorescence 、 Quantum dot 、 Circular dichroism 、 Human serum albumin 、 Chemistry 、 Binding constant 、 Quenching (fluorescence) 、 Spectroscopy 、 Physical chemistry 、 Aqueous solution 、 Analytical chemistry
摘要: Abstract CdTe quantum dots (QDs) were synthesized in aqueous solution using 2-mercaptoethylamine hydrochloride (CA) as stabilizing agents. The interaction between CA-CdTe QDs with human serum albumin (HSA) was investigated by ultraviolet–visible (UV–vis) absorption, circular dichroism (CD), fluorescence and resonance Rayleigh scattering (RRS) spectroscopy. CD spectra revealed that the conformation microenvironment of HSA changed presence QDs. could strongly quench intrinsic static quenching. electrostatic plays a major role complex. binding site number n apparent constant K , corresponding thermodynamic parameters Δ G °, H ° S at different temperatures calculated. led to remarkable enhancement RRS enchantments proportional concentration range 0.05 × 10 −7 4.0 × 10 mol L −1 detection limit 4.2 × 10 −9 . Based on this, methods determinate developed. influences factors some foreign substances all examined, which showed method had good sensitivity selectivity. In addition, reasons for investigated.
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