Adenoviral-mediated p53 tumor suppressor gene therapy of human ovarian carcinoma.

摘要: Mutations of p53 gene are reported in 50-60% human cancers and reintroduction wild-type can suppress cell proliferation. In this study, replication deficient recombinant adenovirus encoding (ACN53) under the control cytomegalovirus (CMV) promoter was constructed. A specific incorporation with ACN53 reduced 3 (deleted gene) cells observed. colony-forming ability SK-OV-3 72-216 h after single infection. highly aggressive ovarian xenograft model established which animals die between 25-45 days. localization study adenovirus-containing beta galactosidase reporter showed effective transfer tumor tissues. Ex vivo treatment followed by injection into nude mice, increased survival treated mice more than 50% compared animals. Gene therapy intraperitoneal two independent experiments revealed that there were some long-term survivors group [2/5 (66 120 days) [2/8 (166 423 days)] ACN53. These findings demonstrate potential suppressor carcinoma.