In silico identification and characterization of novel microsatellite loci for the Blue-and-yellow Macaw Ara ararauna (Linnaeus, 1758) (Psittaciformes, Psittacidae).

作者: Cássia Alves Lima-Rezende , Gislaine Aparecida Fernandes , Helder Elias da Silva , Sarah Dobkowski-Marinho , Victor Fernandes Santos

DOI: 10.1590/1678-4685-GMB-2017-0338

关键词: PsittaciformesThreatened speciesMicrosatelliteEvolutionary biologyAra araraunaPopulationBiologyLocus (genetics)MacawPsittacidae

摘要: The illegal trade is a major threat to many bird species, and parrots are common victims of this activity. Domestic international pet markets interested on different parrot such as the Blue-and-yellow Macaw (Ara ararauna). This South American macaw not globally threatened, but under protection from over-exploitation. study aimed identify characterize novel microsatellite loci for population parentage analysis A. ararauna. Scaffold sequences Ara macao available in NCBI database were used searches using MsatCommander software. We tested total 28 loci, which 25 polymorphic, one was monomorphic, two did generated amplification products. For polymorphic mean number alleles 8.24 (4 - 15 per locus), observed heterozygosity ranged 0.333 0.917, expected 0.353 0.890. paternity exclusion probability identity highly discriminatory. Thus, these markers can be useful assignment tests, helping authorities manage macaws trafficking control commercial breeders.

参考文章(19)
Flavia T. Presti, Adriane P. Wasko, A Review of Microsatellite Markers and their Application on Genetic Diversity Studies in Parrots Open Journal of Genetics. ,vol. 4, pp. 69- 77 ,(2014) , 10.4236/OJGEN.2014.42010
Francois Rousset, None, genepop'007: a complete re-implementation of the genepop software for Windows and Linux Molecular Ecology Resources. ,vol. 8, pp. 103- 106 ,(2008) , 10.1111/J.1471-8286.2007.01931.X
R. Caparroz, C. Y. Miyaki, A. J. Baker, Characterization of microsatellite loci in the Blue‐and‐gold Macaw, Ara ararauna (Psittaciformes: Aves) Molecular Ecology Notes. ,vol. 3, pp. 441- 443 ,(2003) , 10.1046/J.1471-8286.2003.00475.X
Cock Van Oosterhout, William F Hutchinson, Derek PM Wills, Peter Shipley, None, Micro-Checker: Software for identifying and correcting genotyping errors in microsatellite data Molecular Ecology Notes. ,vol. 4, pp. 535- 538 ,(2004) , 10.1111/J.1471-8286.2004.00684.X
Stephen F. Pires, The illegal parrot trade: a literature review Global Crime. ,vol. 13, pp. 176- 190 ,(2012) , 10.1080/17440572.2012.700180
Christopher M. Seabury, Scot E. Dowd, Paul M. Seabury, Terje Raudsepp, Donald J. Brightsmith, Poul Liboriussen, Yvette Halley, Colleen A. Fisher, Elaine Owens, Ganesh Viswanathan, Ian R. Tizard, A Multi-Platform Draft de novo Genome Assembly and Comparative Analysis for the Scarlet Macaw (Ara macao) PLoS ONE. ,vol. 8, pp. e62415- ,(2013) , 10.1371/JOURNAL.PONE.0062415
Markus Schuelke, An economic method for the fluorescent labeling of PCR fragments Nature Biotechnology. ,vol. 18, pp. 233- 234 ,(2000) , 10.1038/72708
Christina Backes, Alexander Rurainski, Gunnar W. Klau, Oliver Müller, Daniel Stöckel, Andreas Gerasch, Jan Küntzer, Daniela Maisel, Nicole Ludwig, Matthias Hein, Andreas Keller, Helmut Burtscher, Michael Kaufmann, Eckart Meese, Hans-Peter Lenhof, An integer linear programming approach for finding deregulated subgraphs in regulatory networks Nucleic Acids Research. ,vol. 40, pp. 1- 13 ,(2012) , 10.1093/NAR/GKR1227