Protein quantification, sandwich ELISA, and real-time PCR used to monitor industrial cleaning procedures for contamination with peanut and celery allergens.

摘要: In the United States, peanut is one of main sources food allergens. Similarly, celery a common allergenic in Western Europe. Severe allergic reactions to both foods are common. Unexpected can occur after consumption celery- and peanut-free as result inadvertent cross-contaminations during manufacturing. Therefore, cooperation with flavor manufacturer, we monitored cleaning process slurry preparation equipment regard contaminations follow-up products compounds. Washing water samples taken different steps were analyzed for presence traces celery-specific real-time polymerase chain reaction (PCR) peanut-specific sandwich enzyme-linked immunosorbent assay (ELISA). PCR ELISA compared nonspecific protein evaluate whether detection be fast cost-effective method monitoring effectiveness wet procedures. Additionally, potential mush, which used source material, measured by mediator release using rat basophilic leukemia (RBL) cell line. conclusion, quantification total washing was suitable process. Our study also revealed evidence that, cases where applicable, removed high efficiency.