Schwann cell migration is integrin-dependent and inhibited by astrocyte-produced aggrecan.

摘要: Schwann cells transplantation has considerable promise in spinal cord trauma to bridge the site of injury and for remyelination demyelinating conditions. They support axonal regeneration sprouting by secreting growth factors providing a permissive surface matrix molecules while shielding axons from inhibitory environment central nervous system. However, following show limited migratory ability they are unable intermingle with host astrocytes. This turn leads formation sharp boundary an abrupt transition between cell graft tissue astrocytes, therefore preventing regenerating exiting graft. The objective this study was identify elements on astrocytes involved restricting migration. Using vitro assays migration, we that aggrecan produced is inhibition motility astrocytic monolayers. Knockdown proteoglycan using RNAi or digestion glycosaminglycan chains improves We further mediates its effect disruption integrin function cells, effects can overcome activation integrins.