Exploiting the genome sequence of Streptomyces nodosus for enhanced antibiotic production.

摘要: The genome of the amphotericin producer Streptomyces nodosus was sequenced. A single scaffold 7,714,110 bp obtained. Biosynthetic genes were identified for several natural products including polyketides, peptides, siderophores and terpenes. majority these clusters specified known compounds. Most silent or expressed at low levels unlikely to compete with production. Biosynthesis a skyllamycin analogue activated by introducing expression plasmids containing either gene LuxR transcriptional regulator synthesis acyl moiety lipopeptide. In an attempt boost production, CoA carboxylases, phosphopantetheinyl transferase AmphRIV activator overexpressed, effects on yields investigated. This study provides groundwork metabolic engineering S. strains produce high analogues.