Zerumbone Suppresses Osteopontin-Induced Cell Invasion Through Inhibiting the FAK/AKT/ROCK Pathway in Human Non-Small Cell Lung Cancer A549 Cells
作者： Chi Gu Kang , Hyo-Jeong Lee , Sung-Hoon Kim , Eun-Ok Lee
DOI: 10.1021/ACS.JNATPROD.5B00796
关键词: Cancer 、 Kinase 、 ROCK1 、 Protein kinase B 、 Biology 、 Cell biology 、 Osteopontin 、 Lim kinase 、 A549 cell 、 Cofilin 、 Cancer research
摘要: Lung cancer is the most frequently diagnosed and leading cause of deaths in United States Korea. We have previously demonstrated that osteopontin (OPN) induces cell invasion through inactivating cofilin. Inactivation cofilin mediated by FAK/AKT/Rho-associated kinase (ROCK) pathway human nonsmall lung (NSCLC) cells. Zerumbone (1) has been shown to exert anticancer activities. In this study, whether how 1 affects OPN-induced was determined NSCLC A549 Results from Boyden chamber assays suggested OPN induced cells strongly suppressed activity without affecting viability. Compound effectively inhibited protein expression ROCK1, phosphorylation LIM 2 (LIMK1/2), addition, immunofluorescence staining showed caused a significant increase lamellipodia formation at edge However, dramatically decreased formation. impaired FAK AKT, as Western blot analysis. Taken together, these results suggest causes considerable suppression inhibiting FAK/AKT/ROCK
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