Neutrophil accumulation and activation by homologous IL-8 in rabbits. IL-8 induces destruction of cartilage and production of IL-1 and IL-1 receptor antagonist in vivo.

摘要: Whether or not IL-8 attracts T lymphocytes and activates neutrophils in vivo remains unclear. Most studies on function of have been done human heterologous animals. To elucidate the role vivo, we injected homologous into rabbit knee joints investigated inflammatory response. Injection 10 micrograms induced a massive accumulation neutrophils. vitro; however, no appreciable lymphocyte rabbits. Although was reported to induce cartilage destruction when animals, observed that did provoke release neutrophil elastase, leading destruction, An inhibitor against elastase (ONO-5046) prevented cartilage. bioactive immunoreactive IL-1 beta receptor antagonist (IL-1Ra) joint cavity. Immunohistochemistry showed IL-1Ra positive cells were infiltrating leukocytes. In neutrophil-depleted rabbits, far lesser concentrations compared with findings normal Thus, are main producers these cytokines responsible for destruction. addition chemotactic activity, proved neutrophil-activating capability respect induction IL-1Ra.