Antiproliferative Effects of Two Amides, Piperine and Piplartine, from Piper Species

摘要: The present work evaluated the cytotoxicity of piplartine {5,6-dihydro-1-[1-oxo-3-(3,4,5-trimethoxyphenyl)-trans-2-propenyl]-2(1H)pyridinone} and piperine {1-[5-(1,3)-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine}, components obtained from Piper species. substances were tested for their on brine shrimp lethality assay, sea urchin eggs development, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay using tumor cell lines lytic activity mouse erythrocytes. Piperine showed higher toxicity in (DL50 = 2.8 +/- 0.3 microg/ml) than 32.3 3.4 microg/ml). Both inhibited development during all phases examined, first third cleavage blastulae, but this was more potent piperine. In MTT most active with IC50 values range 0.7 to 1.7 microg/ml. None induced hemolysis erythrocytes, suggesting that not related membrane damage.