The σ70 Subunit of RNA Polymerase Is Contacted by the λQ Antiterminator during Early Elongation
作者： Bryce E Nickels , Christine W Roberts , Haitao Sun , Jeffrey W Roberts , Ann Hochschild
DOI: 10.1016/S1097-2765(02)00648-2
关键词: Elongation 、 Transcription (biology) 、 Biology 、 Bacteriophage 、 Molecular biology 、 RNA polymerase 、 Biophysics 、 Protein subunit 、 Cell biology
摘要: Abstract The Q protein of bacteriophage λ is a transcription antiterminator that modifies the elongation properties E. coli RNA polymerase (RNAP). To do this, DNA-bound λQ must first engage paused complex. Here we show this engagement with RNAP involves an interaction between and σ 70 , demonstrating can be target regulation during elongation. Furthermore, provide evidence stabilizes conformation requires disengagement segment from core enzyme. Recent structure-based models posit transition initiation to phase staged displacement core. Our findings support for proposal.
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K. Liu, Y. Zhang, K. Severinov, A. Das, M. M. Hanna, Role of Escherichia coli RNA polymerase alpha subunit in modulation of pausing, termination and anti-termination by the transcription elongation factor NusA. The EMBO Journal. ,vol. 15, pp. 150- 161 ,(1996) , 10.1002/J.1460-2075.1996.TB00343.X
Hong Tang, Younggyu Kim, Konstantine Severinov, Alex Goldfarb, Richard H. Ebright, Escherichia coli RNA polymerase holoenzyme: rapid reconstitution from recombinant alpha, beta, beta', and sigma subunits. Methods in Enzymology. ,vol. 273, pp. 130- 134 ,(1996) , 10.1016/S0076-6879(96)73012-4
Jeffrey H Miller, Experiments in molecular genetics ,(1972)
M Lonetto, M Gribskov, C A Gross, The sigma 70 family: sequence conservation and evolutionary relationships. Journal of Bacteriology. ,vol. 174, pp. 3843- 3849 ,(1992) , 10.1128/JB.174.12.3843-3849.1992
K. Kuznedelov, A Role for Interaction of the RNA Polymerase Flap Domain with the sigma Subunit in Promoter Recognition Science. ,vol. 295, pp. 855- 857 ,(2002) , 10.1126/SCIENCE.1066303
J. Joung, D. Koepp, A Hochschild, Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein Science. ,vol. 265, pp. 1863- 1866 ,(1994) , 10.1126/SCIENCE.8091212
Jeffrey W Roberts, Christine W Roberts, Base-Specific Recognition of the Nontemplate Strand of Promoter DNA by E. coli RNA Polymerase Cell. ,vol. 86, pp. 495- 501 ,(1996) , 10.1016/S0092-8674(00)80122-1
K. S. Murakami, Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution. Science. ,vol. 296, pp. 1280- 1284 ,(2002) , 10.1126/SCIENCE.1069594
S. L. Dove, F. W. Huang, A. Hochschild, Mechanism for a transcriptional activator that works at the isomerization step Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 13215- 13220 ,(2000) , 10.1073/PNAS.97.24.13215
E GRAYHACK, X YANG, L LAU, J ROBERTS, Phage lambda gene Q antiterminator recognizes RNA polymerase near the promoter and accelerates it through a pause site Cell. ,vol. 42, pp. 259- 269 ,(1985) , 10.1016/S0092-8674(85)80121-5