Regulation of Innate Lymphoid Cells in Acute Kidney Injury: Crosstalk between Cannabidiol and GILZ.

摘要: Innate lymphoid cells (ILCs) have emerged as largely tissue-resident archetypal of the immune system. We tested hypotheses that renal ischemia-reperfusion injury (IRI) is a contributing factor to polarization ILCs and glucocorticoid-induced leucine zipper (GILZ) cannabidiol regulate them in this condition. Mice subjected unilateral IRI were treated with following agents before restoration blood flow: cannabidiol, DMSO, transactivator transcription- (TAT-) GILZ, or TAT peptide. Thereafter, kidney prepared for flow cytometry analyses. Sham kidneys either TAT-GILZ displayed similar frequencies each subset compared DMSO TAT, respectively. Renal increased ILC1s ILC3s but reduced ILC2s sham group. Cannabidiol treatment reversed pattern evidenced by their DMSO- TAT-treated counterparts. While did not significantly affect positive cannabinoid receptors subtype 2 (CB2+), frequency both CB2+ GILZ-positive (GILZ+) kidneys. Subsequent studies showed GILZ+ subsets ILCs, an effect less marked ILC2s. Treatment was more Indeed, Collectively, results indicate GILZ ILC phenotype, acute injury, effects likely relate modulation endogenous GILZ.