Simple and rapid analysis of four amphetamines in human whole blood and urine using liquid–liquid extraction without evaporation/derivatization and gas chromatography–mass spectrometry

摘要: We developed a simple and rapid analysis method for the determination of four amphetamines in human whole blood urine using gas chromatography–mass spectrometry (GC–MS). During course sample preparation, evaporation process was found to be weak point amphetamine methamphetamine analysis. Liquid–liquid extraction this study conducted without step, upper solvent layer injected directly derivatization, which effectively prevented volatile from vanishing during guaranteed linearity calibration curves. This showed good selectivity, precision, accuracy, at same time fast. The limits detection were 5 ng/ml. As an application study, drug abuse suspect analyzed. may able meet need accurate confirmation positive immunoassay results as well quantification clinical forensic toxicological cases. To our knowledge, no toxicologists have noted that such basic method, or is useful routine GC–MS.