The short- and long-term effects of two isomers of DDT and their metabolite DDE on hormone secretion and survival of human choriocarcinoma JEG-3 cells.
作者： Anna K Wójtowicz , Ewa L Gregoraszczuk , Katarzyna Augustowska
DOI:
关键词: Human chorionic gonadotropin 、 Secretion 、 Apoptosis 、 Internal medicine 、 Endocrinology 、 Chemistry 、 Progesterone secretion 、 Choriocarcinoma 、 Hormone 、 Caspase 3 、 Metabolite
摘要: JEG-3 cells were used to compare the effects of two isomers DDT (1,1,1,-trichloro-2,2-bis(p-chlorophenyl)ethane), p,p'-DDT and o,p'-DDT their metabolite DDE (1,1,-dichloro-2,2-bis(p-chlorophenyl)ethylene) on progesterone (P4) human chorionic gonadotropin (hCG) secretion cell apoptosis. Cells treated with 1, 10, 100 ng/ml or 1 mug/ml each compound for 24 72 h. Twenty four hours exposure at decreased, whereas both DDEs, all investigated concentrations, increased P4 secretion. Seventy two-hour concentrations stimulated Statistically significant decrease in hCG after h increase compounds was noted. Decrease caspase-3 activity observed exposed its metabolites. These findings indicate that are able alter main placental hormone production survival concentration- time-dependent manner.
参考文章(59)
Terry M. Mayhew, Villous trophoblast of human placenta: a coherent view of its turnover, repair and contributions to villous development and maturation. Histology and Histopathology. ,vol. 16, pp. 1213- 1224 ,(2001) , 10.14670/HH-16.1213
Frederick W. Kutz, Patricia H. Wood, David P. Bottimore, Organochlorine Pesticides and Polychlorinated Biphenyls in Human Adipose Tissue* Reviews of Environmental Contamination and Toxicology. ,vol. 120, pp. 1- 82 ,(1991) , 10.1007/978-1-4612-3080-9_1
Régine Sitruk-Ware, Jean-Christophe Thalabard, Tu Lan De Plunkett, Fanny Lewin, Sylvie Epelboin, Irène Mowszowicz, Halina Yaneva, Michel Tournaire, Jacques Chavinie, Pierre Mauvais-Jarvis, Irving M. Spitz, The use of the antiprogestin RU486 (mifepristone) as an abortifacient in early pregnancy--clinical and pathological findings; predictive factors for efficacy. Contraception. ,vol. 41, pp. 221- 243 ,(1990) , 10.1016/0010-7824(90)90065-4
Susan C. Wright, Jian Zhong, James W. Larrick, Inhibition of apoptosis as a mechanism of tumor promotion. The FASEB Journal. ,vol. 8, pp. 654- 660 ,(1994) , 10.1096/FASEBJ.8.9.8005393
J. F. Jarrell, J. Kohut, W. Wrixon, C. G. Zouves, S. Bartlett, D. Villeneuve, C. Franklin, Contamination of human ovarian follicular fluid and serum by chlorinated organic compounds in three Canadian cities Canadian Medical Association Journal. ,vol. 148, pp. 1321- 1327 ,(1993)
T W Clarkson, Environmental contaminants in the food chain The American Journal of Clinical Nutrition. ,vol. 61, ,(1995) , 10.1093/AJCN/61.3.682S
Jessie L. S. Au, M. Guill Wientjes, Rajee R. Kumar, Dong Li, Kinetics of hallmark biochemical changes in paclitaxel-induced apoptosis Aaps Pharmsci. ,vol. 1, pp. 7- 14 ,(1999) , 10.1208/PS010308
Eiji Katsura, Akio Kanetoshi, Yoshihiro Hori, Kunihiko Kobayashi, Hiroyuki Kojima, Mitsuru Iida, Effects of a diphenyl ether-type herbicide, chlornitrofen, and its amino derivative on androgen and estrogen receptor activities. Environmental Health Perspectives. ,vol. 111, pp. 497- 502 ,(2003) , 10.1289/EHP.5724
Rebecca A James, Irva Hertz-Picciotto, Eric Willman, Jean A Keller, M Judith Charles, Determinants of serum polychlorinated biphenyls and organochlorine pesticides measured in women from the child health and development study cohort, 1963-1967. Environmental Health Perspectives. ,vol. 110, pp. 617- 624 ,(2002) , 10.1289/EHP.02110617
J Payne, M Scholze, A Kortenkamp, Mixtures of four organochlorines enhance human breast cancer cell proliferation. Environmental Health Perspectives. ,vol. 109, pp. 391- 397 ,(2001) , 10.1289/EHP.01109391