A novel label free long non-coding RNA electrochemical biosensor based on green L-cysteine electrodeposition and Au–Rh hollow nanospheres as tags

摘要: Nuclear paraspeckle assembly transcript 1 (NEAT1) that reflects whether the body's anti-HIV virus immunity is strong or weak one of many long non-coding RNAs (lncRNAs). The effective detection NEAT1 has great scientific significance and clinical value. However, lncRNA difficult with existing methods. quantification by electrochemical methods based on changes in circuit properties such as capacitance, conductance resistance may solve this problem. In study, we describe a new label-free strategy catalytic signal amplification single-wall carbon nanotubes wrapped Au–Rh hollow nanospheres (Au/Rh-HNP@SWCNT). This useful for detecting NEAT1. Firstly, L-cysteine (L-Cys) was electrodeposited onto Au electrode incubated colloidal to fully expose all binding sites particles, which can bind L-Cys through its thiol-group. It take advantage each surface nanoparticles bound capture probe, contains (GGG)3 trimer electron mediator hemin. results indicate catalysis noticeably enhanced biosensor provided ultrasensitive linear calibration ranged from fM mL−1 100 nM mL−1, limit 0.8863 mL−1. Au/Rh-HNP@SWCNT complex an nano-film exhibited acceptable reproducibility clear selectivity. provide alternative HIV diagnosis