作者: D.M. Maurice , P.G. Watson
DOI: 10.1016/S0014-4835(65)80052-5
关键词: Cornea 、 Staining 、 Albumin 、 Serum albumin 、 Stroma 、 Agar gel 、 Protein concentration 、 Chromatography 、 Biology 、 Electrophoresis 、 Ophthalmology 、 Sensory Systems 、 Cellular and Molecular Neuroscience
摘要: A gradient in concentration of albumin from the centre to periphery rabbit's cornea has been shown by imprinting a strip bare stroma on agar gel and then separating proteins electrophoresis staining them. Measurements indicate that protein at is one-third limbus, average. The reality this distribution was confirmed elution pieces tissue cut cornea. limbal be about one-fifth blood these experiments. similar observed calculated basis enters peripheral capillaries diffuses toward while being slowly lost across corneal surfaces. rate which it surfaces, necessary for making calculation, estimated using 131 l-labelled albumin.