Radioiodide induces apoptosis in human thyroid tissue in culture

摘要: Radioiodide ((131)I) is routinely used for the treatment of toxic adenoma, Graves' disease, and ablation thyroid remnant after thyroidectomy in patients with cancer. The effects ionizing radiations on living cells can be mediated by a necrotic and/or apoptotic process. involvement apoptosis radiation-induced cell death thyrocytes has been questioned. knowledge mechanisms that underlie thyrocyte response to help achieve successful lowest (131)I dose. We developed method study human tissue culture, which we demonstrated induces apoptosis. Human tissues about 1 mm(3) were cultured vitro viability was determined up 3 weeks MTT assay. added culture medium actively taken tissues. occurrence assessed measuring production caspase-cleavage fragment cytokeratin 18 (M30) an enzyme-linked immunoassay. Neither variation number nor spontaneous revealed week culture. induced dose-dependent time-dependent generation M30 fragment. process confirmed caspase-3 PARP cleavage products. These results demonstrate thyrocytes. cultures may useful investigate pathways (131)I.