The influence of cholesterol on melittin lipidation in neutral membranes.
作者： Hannah M. Britt , Jackie A. Mosely , John M. Sanderson
DOI: 10.1039/C8CP06661B
关键词: Cholesterol 、 Melittin 、 Reactive intermediate 、 Biophysics 、 Peptide 、 Bilayer 、 Chemistry 、 POPC 、 Lipid-anchored protein 、 Membrane
摘要: The effects of cholesterol on the process intrinsic lipidation, whereby an acyl chain is transferred from a lipid as donor to membrane-associated acceptor molecule, have been explored using melittin acceptor. Membranes comprising lipids with saturated chains (1,2-dipalmitoyl sn-glycero-3-phosphocholine, DPPC; 1,2-dimyristoyl DMPC) yielded no transfer, whereas membranes composed unsaturated (1,2-dioleoyl DOPC; 1-palmitoyl-2-oleoyl POPC) produced detectable lipidation activity. For all lipids, inclusion led significant increase in activity, greatest effect observed for 20 mol% POPC. In case POPC, also small changes selectivity transfer sn-1 vs. sn-2 positions lipid. Qualitatively, fluid membranes, trend activity exhibits positive correlation bending modulus bilayer and accounted terms penetration depth peptide. Access water reactive intermediates has potential influence rates.
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