Complexation of fluoroquinolone antibiotics with human serum albumin: A fluorescence quenching study
作者： Neelam Seedher , Pooja Agarwal
DOI: 10.1016/J.JLUMIN.2010.04.020
关键词: Stereochemistry 、 Quenching (fluorescence) 、 Ciprofloxacin Hydrochloride 、 Sparfloxacin 、 Nuclear chemistry 、 Fluorescence spectrometry 、 Chemistry 、 Hydrophobic effect 、 Enrofloxacin 、 Human serum albumin 、 Antibacterial agent
摘要: Mechanism of interaction and detailed physico-chemical characterization the binding four fluoroquinolones: levofloxacin, sparfloxacin, ciprofloxacin HCl enrofloxacin with human serum albumin has been studied at physiological pH (7.4) using fluorescence spectroscopic technique. The stoichiometry was found to be 1:1 for all drugs used. association constants were order 104 in most cases. At low drug:protein ratios, a significant fraction added drug bound. predominant interactions involved are hydrogen bonding Van der Waal’s case hydrophobic hydrochloride bonding, electrostatic sparfloxacin. The region did not coincide that probe, 1-anilinonaphthalene-8-sulfonate (ANS). From displacement site-specific probes site-marker drugs, it concluded is site II-specific while I-specific drug. Levofloxacin binds both I II equal affinity. Sparfloxacin had higher affinity than I. It also possible sparfloxacin interface between II. Stern–Volmer analysis data showed quenching mechanism predominantly collisional static mechanisms operative levofloxacin sparfloxacin. High magnitude rate constant process entirely diffusion controlled. Circular dichroism (CD) studies presence cause any major changes secondary structure HSA.
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