Bioconjugation of InGaP quantum dots for molecular sensing
作者： Parveen Kumar , Akash Deep , Sukesh Chander Sharma , Lalit M. Bharadwaj
关键词: Bioconjugation 、 Nanotechnology 、 Bovine serum albumin 、 Surface modification 、 Fluorescence 、 Biosensor 、 Quantum dot 、 Chemistry 、 Serum albumin 、 Surface plasmon resonance
摘要: Abstract Fluorescence-based molecular sensing and cellular imaging are commonly carried out with the application of organic dyes. Quantum dots (QDs) now recognized as better tools because they brighter, size tunable, more photostable than Most proposed QD-based biosensing systems involve elements known toxicity. The present work reports functionalization biocompatible InGaP/ZnS core–shell QDs anti-bovine serum albumin (anti-BSA) to exploit them fluorescent probes for antigen detection. Successful bioconjugation was characterized absorption emission spectra showing blue shifts around 40 30 nm, respectively. Gel electrophoresis particle distribution studies further confirmed mass increment after their anti-BSA. Surface plasmon resonance spectrometry has been used study affinity QD-(anti-BSA) bovine (BSA). Photoluminescence quenching developed probe is observed in presence BSA.
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He Meng, Ji-Yao Chen, Lan Mi, Pei-Nan Wang, Mei-Ying Ge, Yang Yue, Ning Dai, Conjugates of folic acids with BSA-coated quantum dots for cancer cell targeting and imaging by single-photon and two-photon excitation. Journal of Biological Inorganic Chemistry. ,vol. 16, pp. 117- 123 ,(2011) , 10.1007/S00775-010-0709-Z
Ron Gill, Itamar Willner, Itzhak Shweky, Uri Banin, Fluorescence resonance energy transfer in CdSe/ZnS-DNA conjugates: probing hybridization and DNA cleavage. Journal of Physical Chemistry B. ,vol. 109, pp. 23715- 23719 ,(2005) , 10.1021/JP054874P
Catherine A. Royer, Probing protein folding and conformational transitions with fluorescence. Chemical Reviews. ,vol. 106, pp. 1769- 1784 ,(2006) , 10.1021/CR0404390
Shu Xu, Sandeep Kumar, Thomas Nann, Rapid synthesis of high-quality InP nanocrystals. Journal of the American Chemical Society. ,vol. 128, pp. 1054- 1055 ,(2006) , 10.1021/JA057676K
A.C. Tedesco, D.M. Oliveira, Z.G.M. Lacava, R.B. Azevedo, E.C.D. Lima, P.C. Morais, Investigation of the binding constant and stoichiometry of biocompatible cobalt ferrite-based magnetic fluids to serum albumin Journal of Magnetism and Magnetic Materials. ,vol. 272, pp. 2404- 2405 ,(2004) , 10.1016/J.JMMM.2003.12.375
Robert E. Bailey, Andrew M. Smith, Shuming Nie, Quantum dots in biology and medicine Physica E-low-dimensional Systems & Nanostructures. ,vol. 25, pp. 1- 12 ,(2004) , 10.1016/J.PHYSE.2004.07.013
Ming-Shu Hsieh, Nion-Heng Shiao, Wen-Hsiung Chan, Cytotoxic Effects of CdSe Quantum Dots on Maturation of Mouse Oocytes, Fertilization, and Fetal Development International Journal of Molecular Sciences. ,vol. 10, pp. 2122- 2135 ,(2009) , 10.3390/IJMS10052122
Shaopeng Wang, Natalia Mamedova, Nicholas A. Kotov, Wei Chen, Joe Studer, Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates Nano Letters. ,vol. 2, pp. 817- 822 ,(2002) , 10.1021/NL0255193
Austin M. Derfus, Warren C. W. Chan, Sangeeta N. Bhatia, Probing the Cytotoxicity Of Semiconductor Quantum Dots. Nano Letters. ,vol. 4, pp. 11- 18 ,(2004) , 10.1021/NL0347334
Shao-Fa Sun, Bo Zhou, Han-Na Hou, Yi Liu, Guang-Ya Xiang, Studies on the interaction between Oxaprozin-E and bovine serum albumin by spectroscopic methods International Journal of Biological Macromolecules. ,vol. 39, pp. 197- 200 ,(2006) , 10.1016/J.IJBIOMAC.2006.03.020