Inhalation of glycopyrronium inhibits cigarette smoke-induced acute lung inflammation in a murine model of COPD
作者： Liang-liang Shen , Ya-nan Liu , Hui-juan Shen , Chong Wen , Yong-liang Jia
DOI: 10.1016/J.INTIMP.2013.12.021
关键词: Inflammation 、 Bronchodilator 、 Bronchoalveolar lavage 、 Monocyte 、 Pharmacology 、 Tumor necrosis factor alpha 、 Interleukin 、 Myeloperoxidase 、 Immunology 、 Medicine 、 COPD
摘要: Glycopyrronium bromide (GB) is a muscarinic receptor antagonist that has been used as long-acting bronchodilator in chronic obstructive pulmonary disease (COPD) patients. The aim of this study was to investigate the anti-inflammatory activity inhaled GB cigarette smoke-induced acute lung inflammation mouse model. We found aerosol pre-treatment with suppresses accumulation neutrophils and macrophages bronchoalveolar lavage fluid (BALF) smoke (CS)-exposed mice. at doses 300 600 μg/ml significantly inhibited CS-induced increases mRNA protein expression levels interleukin (IL)-1β, tumor necrosis factor (TNF)-α, monocyte chemotactic (MCP)-1 transforming growth (TGF)-β1 tissues BALF. Moreover, dose changes glutathione (GSH) myeloperoxidase (MPO) activities BALF, decreased matrix metalloproteinases (MMP)-9, increased tissue inhibitor (TIMP)-1, determined through immunohistochemical staining tissue. Our results demonstrate beneficial effects on inflammatory reaction COPD.
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