Nanosensor electrical immunoassay for quantitative detection of NT-pro brain natriuretic peptide.
作者： Anjan Panneer Selvam , Shalini Prasad
DOI: 10.2217/FCA.12.76
关键词: Immunoassay 、 Nanosensor 、 Detection limit 、 Aluminum oxide 、 Nanoporous 、 Natriuretic peptide 、 Medicine 、 Chromatography 、 Pro-Brain Natriuretic Peptide 、 Dielectric spectroscopy
摘要: Aim: To demonstrate a label-free electrical immunoassay for profiling vascular biomarker N-terminal pro-brain natriuretic peptide (NT-proBNP) associated with improved cardiac risk prediction. Materials & methods: A high-density nanowell-based has been designed by integrating nanoporous aluminum oxide onto printed circuit board chips the detection of NT-proBNP. The concentration is quantitatively determined measuring impedance changes to double layer within nanowells using electrochemical spectroscopy. Detection sensitivity in fg/ml range was obtained due spatial confinement target biomarkers size-matched nanowells. Results discussion: Electrical performance NT-proBNP phosphate-buffered saline (PBS) and human serum (HS). lower limit sensor observed be 10 PBS 500 HS. upper to...
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