Herpes simplex virus (HSV)-mediated ICAM-1 gene transfer abrogates tumorigenicity and induces anti-tumor immunity.

摘要: Costimulatory and cellular adhesion molecules are thought to be essential components of antigen presentation in the immune response cancer. The current studies examine gene transfer utilizing herpes viral amplicon vectors (HSV) direct surface expression molecules, specifically evaluate potential a tumor-expressing intercellular molecule-1 (ICAM-1) elicit an anti-tumor response. human ICAM-1 (hICAMI) was inserted into HSV vector tested transplantable rat hepatocellular carcinoma colorectal cancer cell line. Cell assessed by flow cytometry. Lymphocyte binding HSV-hICAM1-transduced cells compared with that transduced not carrying ICAM gene. Tumorigenicity tumor were syngeneic Buffalo rats. Additionally, immunization irradiated (10,000 rads) performed determine its effect on growth. A 20-min exposure at multiplicity infection (MOI) 1 resulted high-level approximately 25% cells. Transduced or exhibited significantly enhanced lymphocytes (p < 0.05). elicited increase infiltration CD4+ vivo decreased tumorigenicity. Immunization protected against growth subsequent injected parental amplicon-mediated is efficient method for modifying molecules. Increased represents promising anti-cancer strategy.