Direct-Double-Differential PCR for Gene Dosage Quantification of c-myc

摘要: c- myc gene amplification that leads to overexpression has been shown play a major role in cancer development, especially breast (1). Two methodological approaches based on PCR, differential PCR (2) and competitive (3), have developed for dosage quantification applied . When these methods are used, both degraded DNA the necessarily low competitor concentrations, which prone dilution errors, lead over- or underestimation of dosages. Methods using separate comparisons two single-copy reference genes any sample (double-differential PCR) (4)(5), series competitors (competitive-differential (6), (in (7) introduced quantitative circumvent those problems. In consequence, labor-intensive, time-consuming, expensive. We improved reliable sensitive direct-double-differential method fragments different genes, manganese superoxide dismutase (SOD2) β-globin (HBB) , target fragment (c- ) coamplified simultaneously one reaction tube. The product …