Peroxisome size provides insights into the function of autophagy-related proteins.

摘要: Autophagy is a major pathway of intracellular degradation mediated by formation autophagosomes. Recently, autophagy was implicated in the bacteria, whose size often exceeds capacity normal However, adaptations autophagic machinery for sequestration large cargos were unknown. Here we developed yeast model system to study effect cargo on requirement autophagy-related (Atg) proteins. We controlled peroxisomes before their turnover pexophagy, selective peroxisomes, and found that peroxisome determines Atg11 Atg26. Small can be degraded without these Atg26 becomes essential medium peroxisomes. Additionally, pexophagy-specific phagophore assembly site, organized dual interaction Atg30 with functionally active Atg17, In contrast, Atg28 partially required all pathways independent size, suggesting it component core machinery. As rule, larger cargo, more cargo-specific Atg proteins become its sequestration.