An archaeal protein homologous to mammalian SRP54 and bacterial Ffh recognizes a highly conserved region of SRP RNA
作者： Hideki Maeshima , Emi Okuno , Tadanori Aimi , Tsutomu Morinaga , Takuzi Itoh
DOI: 10.1016/S0014-5793(01)02996-9
关键词: Pyrococcus furiosus 、 Nuclease protection assay 、 5.8S ribosomal RNA 、 RNA-binding protein 、 RNA editing 、 Biochemistry 、 Molecular biology 、 Non-coding RNA 、 Biology 、 Signal recognition particle RNA 、 RNA
摘要: The gene encoding the 54 kDa protein of signal recognition particle (SRP54) in hyperthermophilic archaeon Pyrococcus furiosus has been cloned and sequenced. Recombinant P. SRP54 (pf-SRP54) N-terminal G-domain C-terminal M-domain (pf-SRP54M) pf-SRP54 with an amino-terminal addition six histidine residues were expressed Escherichia coli subjected to binding experiments for SRP RNA, non-conserved 213-nucleotide RNA (helices 1, 2, 3, 4 5) conserved 107-nucleotide 6 8) from RNA. properties purified determined by filter assays. histidine-tagged pf-SRP54M bound specifically absence pf-SRP19, unlike eukaryotic homologue, apparent constant (K) 18 nM.
elsevier.com
sci-hub.se 参考文章(32)
Takuzi Itoh, Primary structure of an acidic ribosomal protein YPA1 from Saccharomyces cerevisiae: Isolation and characterization of peptides and the complete amino acid sequence Biochimica et Biophysica Acta. ,vol. 671, pp. 16- 24 ,(1981) , 10.1016/0005-2795(81)90088-X
Christian W Zwieb, Kimberly Chittenden, Shaun D. Black, Systematic site-directed mutagenesis of protein SRP19. Identification of the residues essential for binding to signal recognition particle RNA. Journal of Biological Chemistry. ,vol. 269, pp. 20497- 20502 ,(1994) , 10.1016/S0021-9258(17)32020-3
Hiromi Furumoto, Atsuo Taguchi, Takashi Itoh, Tsutomu Morinaga, Takuzi Itoh, 5S rRNA binding proteins from the hyperthermophilic archaeon, Pyrococcus furiosus. FEBS Letters. ,vol. 486, pp. 195- 199 ,(2000) , 10.1016/S0014-5793(00)02293-6
Krishne Gowda, Kimberly Chittenden, Christian Zwieb, Binding site of the M-domain of human protein SRP54 determined by systematic site-directed mutagenesis of signal recognition particle RNA Nucleic Acids Research. ,vol. 25, pp. 388- 394 ,(1997) , 10.1093/NAR/25.2.388
P Walter, G Blobel, Translocation of proteins across the endoplasmic reticulum. II. Signal recognition protein (SRP) mediates the selective binding to microsomal membranes of in-vitro-assembled polysomes synthesizing secretory protein. Journal of Cell Biology. ,vol. 91, pp. 551- 556 ,(1981) , 10.1083/JCB.91.2.551
Kazuyoshi Sano, Atsuo Taguchi, Hiromi Furumoto, Taizo Uda, Takuzi Itoh, Cloning, sequencing, and characterization of ribosomal protein and RNA polymerase genes from the region analogous to the alpha-operon of escherichia coli in halophilic archaea, halobacterium halobium. Biochemical and Biophysical Research Communications. ,vol. 264, pp. 24- 28 ,(1999) , 10.1006/BBRC.1999.1480
Yoshiko Hasegawa, Nozomi Sawaoka, Nozomi Kado, Minako Ochi, Takuzi Itoh, Cloning and sequencing of the homologues of both the bacterial and eukaryotic initiation factor genes (hIF‐2 and heIF‐2γ from archaeal Halobacterium halobium Iubmb Life. ,vol. 46, pp. 495- 507 ,(1998) , 10.1080/15216549800204022
Takayuki Miyokawa, Takeru Urayama, Kumika Shimooka, Takuzi Itoh, Organization and nucleotide sequences of ten ribosomal protein genes from the region equivalent to the S10 operon in the archaebacterium, Halobacterium halobium. Iubmb Life. ,vol. 39, pp. 1209- 1220 ,(1996) , 10.1080/15216549600201402
John L. Diener, Charles Wilson, Role of SRP19 in assembly of the Archaeoglobus fulgidus signal recognition particle. Biochemistry. ,vol. 39, pp. 12862- 12874 ,(2000) , 10.1021/BI001180S
Takuzi ITOH, Complete nucleotide sequence of the ribosomal ‘A’ protein operon from the archaebacterium, Halobacterium halobium FEBS Journal. ,vol. 176, pp. 297- 303 ,(1988) , 10.1111/J.1432-1033.1988.TB14281.X