Resistance of Undisturbed Soil Microbiomes to Ceftriaxone Indicates Extended Spectrum β-Lactamase Activity
作者： Joao Gatica , Kun Yang , Eulyn Pagaling , Edouard Jurkevitch , Tao Yan
关键词: Escherichia coli 、 Ceftriaxone 、 Biological activity 、 Soil water 、 Antibiotic resistance 、 Microcosm 、 Cephalosporin 、 Microbiology 、 Antibiotics 、 Biology
摘要: Emergence and spread of antibiotic resistance, specifically resistance to third generation cephalosporins associated with extended spectrum β-lactamase (ESBL) activity, is one the greatest epidemiological challenges our time. In this study we addressed impact cephalosporin ceftriaxone on microbial activity bacterial community composition two physically chemically distinct undisturbed soils in highly regulated microcosm experiments. Surprisingly, periodical irrigation clinical doses did not affect their activity; only moderately impacted diversity (α β) soils. Corresponding slurry experiments demonstrated that capacity rapidly diminished presence soil, approximately 70% inactivation could be explained by biological activity. The nature degradation soil was supported amended model Escherichia coli strains sterile non-sterile absence ubiquitous ESBL genes (blaTEM, blaCTX-M blaOXA) DNA extracts. Collectively, these results suggest microbiomes stems from even more, broad-spectrum raising questions regarding scope implications ESBLs microbiomes.
frontiersin.org
core.ac.uk
sci-hub.se 参考文章(68)
Johan Schnürer, Thomas Rosswall, Fluorescein Diacetate Hydrolysis as a Measure of Total Microbial Activity in Soil and Litter Applied and Environmental Microbiology. ,vol. 43, pp. 1256- 1261 ,(1982) , 10.1128/AEM.43.6.1256-1261.1982
Z. Khan, H. Al-Sayer, T. Das Chugh, R. Chandy, F. Provost, P. Boiron, Antimicrobial susceptibility profile of soil isolates of Nocardia asteroides from Kuwait Clinical Microbiology and Infection. ,vol. 6, pp. 94- 98 ,(2000) , 10.1046/J.1469-0691.2000.00026.X
K. Kümmerer, A. Henninger, Promoting resistance by the emission of antibiotics from hospitals and households into effluent Clinical Microbiology and Infection. ,vol. 9, pp. 1203- 1214 ,(2003) , 10.1111/J.1469-0691.2003.00739.X
Chu Thi Thanh Binh, Holger Heuer, Martin Kaupenjohann, Kornelia Smalla, Piggery manure used for soil fertilization is a reservoir for transferable antibiotic resistance plasmids. FEMS Microbiology Ecology. ,vol. 66, pp. 25- 37 ,(2008) , 10.1111/J.1574-6941.2008.00526.X
Bing Li, Ying Yang, Liping Ma, Feng Ju, Feng Guo, James M Tiedje, Tong Zhang, Metagenomic and network analysis reveal wide distribution and co-occurrence of environmental antibiotic resistance genes. The ISME Journal. ,vol. 9, pp. 2490- 2502 ,(2015) , 10.1038/ISMEJ.2015.59
Shannon M. Mitchell, Jeffrey L. Ullman, Amy L. Teel, Richard J. Watts, pH and temperature effects on the hydrolysis of three β-lactam antibiotics: Ampicillin, cefalotin and cefoxitin Science of The Total Environment. ,vol. 466, pp. 547- 555 ,(2014) , 10.1016/J.SCITOTENV.2013.06.027
Theresa A. McHugh, George W. Koch, Egbert Schwartz, Minor Changes in Soil Bacterial and Fungal Community Composition Occur in Response to Monsoon Precipitation in a Semiarid Grassland Microbial Ecology. ,vol. 68, pp. 370- 378 ,(2014) , 10.1007/S00248-014-0416-3
Fiona Walsh, Brion Duffy, The Culturable Soil Antibiotic Resistome: A Community of Multi-Drug Resistant Bacteria PLoS ONE. ,vol. 8, pp. e65567- ,(2013) , 10.1371/JOURNAL.PONE.0065567
Johann D.Pitout, Molecular Detection of Bacteria Producing Newer Types of β-Lactamases Current Genomics. ,vol. 7, pp. 171- 177 ,(2006) , 10.2174/138920206777780238
Hua Yang, Oleksandr A. Byelashov, Ifigenia Geornaras, Lawrence D. Goodridge, Kendra K. Nightingale, Keith E. Belk, Gary C. Smith, John N. Sofos, Presence of antibiotic-resistant commensal bacteria in samples from agricultural, city, and national park environments evaluated by standard culture and real-time PCR methods. Canadian Journal of Microbiology. ,vol. 56, pp. 761- 770 ,(2010) , 10.1139/W10-060