pH-change protective PB-b-PEO polymersomes
作者： Waltraut Mueller , Kaloian Koynov , Sebastien Pierrat , Raphael Thiermann , Karl Fischer
DOI: 10.1016/J.POLYMER.2011.01.028
关键词: Fluorescence microscope 、 Chemical engineering 、 Fluorescence 、 Membrane 、 Vesicle 、 Polymersome 、 Dynamic light scattering 、 Phloxine 、 Fluorescence correlation spectroscopy 、 Chromatography 、 Materials science
摘要: Poly(butadiene)-b-poly(ethylene oxide) vesicles were successfully loaded with hydrophilic dye Phloxine B. Dye addition during vesicle formation leads to B encapsulated inside the water filled core as well freely diffusing molecules. The removal of uncapsulated substrate involves time consuming methods like dialysis or harsher ultra filtration selective precipitation, posing risk irreversible sample manipulation. Here used pH sensitive fluorescence indicator allows characterization loading without separation procedure by adjusting value. Additionally membrane blocking efficiency can be studied dependent measurements. Cryogenic TEM studies showed that self-assembled structure remained unchanged when was incorporated within vesicles. Fluorescence microscopy imaging proved encapsulation volume. combination correlation spectroscopy (FCS) and dynamic light scattering (DLS) measurements ensemble confirmed those results additionally.
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