Contrasting effects of cycloheximide on mitochondrial protein synthesis ‘in vivo’ and ‘in vitro’

摘要: Abstract 1. 1) Our results show that cycloheximide at concentrations up to 500 μg./ml. does not inhibit 14C-leucine incorporation into the protein of isolated mitochondria from either rat liver Krebs II mouse ascites tumour cells. 2. 2) However, when cells are labelled ‘ in vivo ’ presence as low 0.25 μg./ml., considerable inhibition 14 C-leucine mitochondrial fraction is observed. Even subfractionated with Triton X-100, both soluble and insoluble fractions still incorporation. 3. 3) Possible explanations for these observations given discussed special reference other workers who have attempted use a selective inhibitor microsomal synthesis.