Detection and mutation of a luxS-encoded autoinducer in Proteus mirabilis.
作者： Rachel Schneider , C. Virginia Lockatell , David Johnson , Robert Belas
DOI: 10.1099/00221287-148-3-773
关键词: Biology 、 Null allele 、 Swarming (honey bee) 、 Autoinducer-2 、 Autoinducer 、 Microbiology 、 Quorum sensing 、 Swarming motility 、 Proteus mirabilis 、 Virulence
摘要: Quorum sensing regulates the expression of virulence factors in a wide variety pathogenic bacteria. This study has shown that Proteus mirabilis harbours homologue luxS, gene required for synthesis quorum autoinducer 2 (AI-2). AI-2 activity is expressed during and correlated with initiation swarming migration on agar surfaces. The P. luxS locus was cloned LuxS− strain constructed by allelic-exchange mutagenesis. While lacking activity, null mutation however, did not affect swimming or motility, swarmer cell differentiation, mouse model ascending urinary tract infection.
sci-hub.st 参考文章(54)
M. Pirhonen, D. Flego, R. Heikinheimo, E.T. Palva, A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora. The EMBO Journal. ,vol. 12, pp. 2467- 2476 ,(1993) , 10.1002/J.1460-2075.1993.TB05901.X
Gary Telford, D. Wheeler, Paul Williams, P. T. Tomkins, P. Appleby, Herbert Sewell, Gordon S. A. B. Stewart, Barrie W. Bycroft, David I. Pritchard, The Pseudomonas aeruginosa Quorum-Sensing Signal Molecule N-(3-Oxododecanoyl)-l-Homoserine Lactone Has Immunomodulatory Activity Infection and Immunity. ,vol. 66, pp. 36- 42 ,(1998) , 10.1128/IAI.66.1.36-42.1998
V de Lorenzo, M Herrero, U Jakubzik, K N Timmis, Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. Journal of Bacteriology. ,vol. 172, pp. 6568- 6572 ,(1990) , 10.1128/JB.172.11.6568-6572.1990
G R Chippendale, J W Warren, A L Trifillis, H L Mobley, Internalization of Proteus mirabilis by human renal epithelial cells. Infection and Immunity. ,vol. 62, pp. 3115- 3121 ,(1994) , 10.1128/IAI.62.8.3115-3121.1994
D E Johnson, R G Russell, C V Lockatell, J C Zulty, J W Warren, H L Mobley, Contribution of Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mouse model of ascending urinary tract infection. Infection and Immunity. ,vol. 61, pp. 2748- 2754 ,(1993) , 10.1128/IAI.61.7.2748-2754.1993
V L Miller, J J Mekalanos, A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. Journal of Bacteriology. ,vol. 170, pp. 2575- 2583 ,(1988) , 10.1128/JB.170.6.2575-2583.1988
R Belas, D Erskine, D Flaherty, Transposon mutagenesis in Proteus mirabilis. Journal of Bacteriology. ,vol. 173, pp. 6289- 6293 ,(1991) , 10.1128/JB.173.19.6289-6293.1991
R Belas, A Mileham, M Simon, M Silverman, Transposon mutagenesis of marine Vibrio spp. Journal of Bacteriology. ,vol. 158, pp. 890- 896 ,(1984) , 10.1128/JB.158.3.890-896.1984
Clive Ariison, Hsin-Chih Lai, Colin Hughes, Co‐ordinate expression of virulence genes during swarm‐cell differentiation and population migration of Proteus mirabilis Molecular Microbiology. ,vol. 6, pp. 1583- 1591 ,(1992) , 10.1111/J.1365-2958.1992.TB00883.X
Simon Swift, John P. Throup, Paul Williams, George P.C. Salmond, Gordon S.A.B. Stewart, Quorum sensing: a population-density component in the determination of bacterial phenotype Trends in Biochemical Sciences. ,vol. 21, pp. 214- 219 ,(1996) , 10.1016/S0968-0004(96)80018-1