Genomic comparison of Yersinia pestis and Yersinia pseudotuberculosis by combination of suppression subtractive hybridization and DNA microarray

作者: Xiaoyi Wang , Dongsheng Zhou , Long Qin , Erhei Dai , Jianguo Zhang

DOI: 10.1007/S00203-006-0129-1

关键词: BiologySyntenyEnterobacteriaceaeSuppression subtractive hybridizationComparative genomic hybridizationDNA microarrayYersinia pestisGenomeYersinia pseudotuberculosisGeneticsMicrobiology

摘要: In order to further figure out the genetic differences between Yersinia pestis and pseudotuberculosis, provide novel insights into evolution of Y. pestis, we compared genomes pseudotuberculosis serogroup I strain ATCC29833 Antiqua 49006 using a combination suppression subtractive hybridization (SSH) comparative genomic with DNAs from diverse panel strains. SSH followed by BLAST analysis revealed 112 fragments specific ATCC29833, sequence data strains CO92, KIM 91001. We identified 17 that appeared be newly determined contents pseudotuberculosis. The microarray showed parallel loss genes contributed greatly not only significant divergence but also intra-species microevolution both species. results confirmed our earlier hypothesis isolates natural plague focus B in China represented most ancestral China, hence phylogenetically closest

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