Fabrication and characterization of soft macroporous Jeffamine cryogels as potential materials for tissue applications

摘要: Macroporous three-dimensional (3D) scaffolds have been proven to function as structural substrates and apertures for cell growth proliferation thereby ensuring tissue regeneration. Such demonstrate high pore interconnectivity functionality a result permit adherence while maintaining mechanical strength, biodegradability biocompatibility without causing any immune response. In this study, macroporous cryogels were synthesized from difunctional Jeffamine (600, 900 2000 g mol−1) glutaraldehyde in the presence of trifunctional (403 crosslinker via freeze-drying technique. The fabricated subsequently reduced by reductive amination create more active functional groups which increase biological interaction. groups, efficacy, size/surface topology thermal stability analysed using FT-IR 13C-NMR, SEM TGA, respectively. Visco-elastic behaviour was investigated unconfined compression test depicted good elastic modulus strength. Considerable swelling ratios obtained alongside vitro degradation analysis performed 10 days at pH 7.4. addition, evaluated MTT assay 24 h 37 °C Human Umbilical Vein Endothelial Cells (HUVEC) cytotoxicity evaluation on non-reduced cryogels.