Evidence that hypoxia-inducible factor-1 (HIF-1) mediates transcriptional activation of interleukin-1β (IL-1β) in astrocyte cultures
作者： Wandong Zhang , Jelena-Mojsilovic Petrovic , Debbie Callaghan , Aimee Jones , Hong Cui
DOI: 10.1016/J.JNEUROIM.2006.01.014
关键词: Regulation of gene expression 、 Glial fibrillary acidic protein 、 Reporter gene 、 Molecular biology 、 Astrocyte 、 Transcription factor 、 Transfection 、 Transactivation 、 Biology 、 Proteasome inhibitor
摘要: … RNA (0.5 μg) was then mixed with 0.5 μg of oligo (dT) 12–18 primers, heated for 10 min at 70 C and … Specific primers for human and mouse IL-1β, human and mouse HIF-1α and the …
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