Surface charge-and space-dependent transport of proteins in crowded environments of nanotailored posts

摘要: The reaction and diffusion of molecules across barriers through crowded environments is integral to biological system function separation technologies. Ordered, microfabricated post arrays are a promising route creating synthetic with controlled chemical physical characteristics. They can be used create environments, mimic aspects cellular membranes serve as engineered replacements polymer-based media. Here, the translational fluorescein isothiocyante various forms green fluorescent protein (GFP), including “supercharged” variants, examined in silicon-based array environment. technique fluorescence recovery after photo-bleaching (FRAP) combined analytical approximations numerical simulations assess relative effects on molecular transport, respectively. FRAP experiments were conducted for 64 different cases where species, spacing density posts, surface charge posts varied. In all cases, dense packing hindered diffusive transport species. supercharged GFPs strongly interacted oppositely charged surfaces. With similar charges, primarily limited by diffusion. For conventional, enhanced GFP positively environment, was coupled action interaction posts. Quantification size-, space-, time-, charge-dependent provide insight into natural processes guide design development selective membrane systems.