Coating of Single DNA Molecules by Genetically Engineered Protein Diblock Copolymers
作者： Armando Hernandez-Garcia , Marc W. T. Werten , Martien Cohen Stuart , Frits A. de Wolf , Renko de Vries
关键词: Dispersity 、 DNA 、 Amino acid 、 Molecule 、 Self-assembly 、 Polymer chemistry 、 Copolymer 、 Materials science 、 Polymer 、 Protein design 、 Biophysics
摘要: Coating DNA is an effective way to modulate its physical properties and interactions. Current chemosynthetic polymers form aggregates with random size shape. In this study, monodisperse protein diblock copolymers are produced at high yield in recombinant yeast. They carry a large hydrophilic colloidal block (≈400 amino acids) linked short binding (≈12 basic acids). It demonstrated that these complex single molecules as highly stable nanorods, reminiscent of cylindrical viruses. proposed inter- intramolecular bridging prevented completely by the small attached stability block. These diblocks serve scaffold can be tuned for application DNA-based nanotechnology.
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