An ultrastructural evaluation of the cell organelle specificity of the uranaffin reaction in two human endocrine neoplasms.

摘要: The organelle specificity of the uranaffin reaction was determined by subjecting two human neoplasms (a pheochromocytoma and islet cell carcinoma) to four different experimental conditions. In Uranaffin Procedure (UP) I, fixed tissue immersed in 0.9% sodium chloride (NaCl) before reacting with 4% uranyl acetate (pH 3.9) for 24 hours. UP II, prepared as I exception that 48 III, 0.1M cacodylate buffer 7.2) instead NaCl. IV, III hours When UPs II were utilized, only three organelles showed electron-dense reactivity: nucleus, ribosomes, cytoplasmic neurosecretory-like granules. nuclear chromatin, nucleolus, interchromatinic granules perichromatinic intensely stained. product all uranaffin-positive had a finely granular appearance. isotonic saline, non-specific reactivity observed. some areas distinct crystalline appearance filled cytosol, cisternae endoplasmic reticulum, Golgi apparatus, perinuclear cisternae, mitochondria, larger lysosome-like bodies. There statistically significant (p less than 0.05) increase number granules/mu2 when both endocrine reacted using did not result an non-specificity staining reaction.