Construction of long DNA molecules using long PCR‐based fusion of several fragments simultaneously
作者： Nikolai A Shevchuk , Anton V Bryksin , Yevgeniya A Nusinovich , Felipe C Cabello , Margaret Sutherland
DOI: 10.1093/NAR/GNH014
关键词: Proofreading 、 Biology 、 DNA 、 Polymerase cycling assembly 、 Genetics 、 Genome 、 Sequence analysis 、 Overlap extension polymerase chain reaction 、 Recombinant DNA 、 Polymerase chain reaction
摘要: … DNA constructs as long as 20 kb is proposed. The method, which we call long multiple fusion, has been used to assemble up to four fragments … 20 kb products (from fragments of 11, 1.7 …
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