The two-component phoR-phoP system of Streptomyces natalensis: Inactivation or deletion of phoP reduces the negative phosphate regulation of pimaricin biosynthesis
作者： Marta V. Mendes , Sedef Tunca , Nuria Antón , Eliseo Recio , Alberto Sola-Landa
DOI: 10.1016/J.YMBEN.2006.10.003
关键词: Biology 、 Biosynthesis 、 Biochemistry 、 Mutant 、 Phosphate 、 Streptomyces natalensis 、 Gene 、 Promoter 、 Yeast 、 Response regulator
摘要: Abstract The biosynthesis of the antifungal pimaricin in Streptomyces natalensis is very sensitive to phosphate regulation. Concentrations inorganic above 1 mM drastically reduced production. At 10 mM phosphate, expression all ( pim ) genes including pathway-specific positive regulator pimR fully repressed. phoU-phoR-phoP cluster S. encoding two-component Pho system was cloned and sequenced. Binding response PhoP consensus PHO boxes phoU-phoRP intergenic promoter region observed. A phoP -disrupted mutant a phoR-phoP deletion were obtained. Production these two mutants increased up 80% complex yeast extract–malt extract (YEME) or NBG media showed sensitivity control. Four genes, pimS1 , pimS4 pimC pimG mutant. However, no found regions any suggesting that control mediated indirectly by PhoR-PhoP involving modification regulators.
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