Metabolic reprogramming in nodules, roots, and leaves of symbiotic soybean in response to iron deficiency.

摘要: To elucidate the mechanism of adaptation leguminous plants to iron (Fe)-deficient environment, comprehensive analyses soybean (Glycine max) (sampled at anthesis) were conducted under Fe-sufficient control and Fe-deficient treatment using metabolomic physiological approach. Our results show that soybeans grown conditions showed lower nitrogen (N) fixation efficiency; however, ureides increased in different tissues, indicating potential N-feedback inhibition. N assimilation was inhibited as observed repressed amino acids biosynthesis reduced proteins roots nodules. In leaves, many increased, accompanied by reduction malate, fumarate, succinate, α-ketoglutarate, which implies reprogramming stimulated anaplerotic pathway. Accordingly, organic nodules; enzymes involved related metabolic pathway (e.g., Krebs cycle) opposite activity between nodules, indicative mechanisms. Sugars or maintained constant level tissues Fe deficiency, probably relates oxidative stress, cell wall damage, feedback regulation. Increased ascorbate, nicotinate, raffinose, galactinol, proline possibly helped resist stress induced deficiency. Overall, deficiency coordinated symbiotic plants.