Regulation of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Activity in Human Fibroblasts by Lipoproteins
作者： M. S. Brown , S. E. Dana , J. L. Goldstein
关键词: 7-Dehydrocholesterol reductase 、 Enzyme activator 、 Enzyme 、 Biosynthesis 、 Reductase 、 Coenzyme A 、 Biology 、 Enzyme assay 、 Biochemistry 、 Cholesterol
摘要: The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.34), the rate-limiting enzyme hepatic cholesterol biosynthesis, is suppressed in human fibroblasts cultured presence serum. This increases by more than 10-fold after removal serum from medium. rise requires de novo protein synthesis and not accompanied changes activities several other cellular enzymes. factor responsible for suppression present sera at least four mammalian species, it found low-density lipoproteins. Human high-density lipoproteins, very lipoproteins chicken egg yolk, fraction containing no do suppress reductase.
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sci-hub.se 参考文章(12)
James C. Warren, Properties of steroid sulphatase and arylsulphatase activities of human placenta. Biochemical Journal. ,vol. 105, pp. 233- 241 ,(1967) , 10.1042/BJ1050233
Petar Alaupovic, Diana M. Lee, Walter J. McConathy, Studies on the composition and structure of plasma lipoproteins Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 260, pp. 689- 707 ,(1972) , 10.1016/0005-2760(72)90018-5
John M. Kissane, Eli Robins, The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry. ,vol. 233, pp. 184- 188 ,(1958) , 10.1016/S0021-9258(19)68051-8
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Robert P. Noble, Electrophoretic separation of plasma lipoproteins in agarose gel Journal of Lipid Research. ,vol. 9, pp. 693- 700 ,(1968) , 10.1016/S0022-2275(20)42680-X
N. W. Seeds, A. G. Gilman, T. Amano, M. W. Nirenberg, Regulation of Axon Formation by Clonal Lines of a Neural Tumor Proceedings of the National Academy of Sciences of the United States of America. ,vol. 66, pp. 160- 167 ,(1970) , 10.1073/PNAS.66.1.160
Lewis A. Hillyard, Harriet M. White, Sharon A. Pangburn, Characterization of apolipoproteins in chicken serum and egg yolk. Biochemistry. ,vol. 11, pp. 511- 518 ,(1972) , 10.1021/BI00754A005
Carolyn D. Williams, Joel Avigan, In vitro effects of serum proteins and lipids on lipid synthesis in human skin fibroblasts and leukocytes grown in culture Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 260, pp. 413- 423 ,(1972) , 10.1016/0005-2760(72)90056-2
Richard J. Havel, Howard A. Eder, Joseph H. Bragdon, THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM Journal of Clinical Investigation. ,vol. 34, pp. 1345- 1353 ,(1955) , 10.1172/JCI103182
Charles M. Radding, Daniel Steinberg, STUDIES ON THE SYNTHESIS AND SECRETION OF SERUM LIPOPROTEINS BY RAT LIVER SLICES Journal of Clinical Investigation. ,vol. 39, pp. 1560- 1569 ,(1960) , 10.1172/JCI104177