DENDRITIC CELLS REGULATE THE INDUCTION OF EFFECTOR AND MEMORY CD8+ T CELLS

摘要: Dendritic cells (DCs) are key antigen-presenting in the immune system that can induce pathogen-specific T cell responses by presenting antigen (signal 1) to antigen-specific combination with co-stimulatory/inhibitory molecules 2) and secretion of cytokines 3). The ability DCs orchestrate CD8+ responses, combined generate high numbers vitro allows for their use DC-based vaccination protocols. success protocols other forms immunotherapy cancer is believed depend on successful induction both effector (CTLs), able migrate into kill tumors, long-lived memory cells, a secondary response upon tumor recurrence. However, signals drive differentiation each these subsets role this respect remain unclear. Studies have suggested same DC early after maturation while inducing prolonged when become exhausted. Here, I analyzed matured under conditions mimicking acute/early inflammation (“inflammatory-DCs”) or chronic/late (“non-inflammatory-DCs”) cells. observed “inflammatory-DCs” produce levels IL-12p70 naive cytolytic peripheral homing ability. Furthermore, demonstrate process. In contrast, “non-inflammatory-DCs” (exhausted DCs) do not direct central-memory superior anti-tumor guided me develop an alternative, low-cost method generating strong CTL Lastly, show modulation tumor-chemokine environment IFN, poly-I:C indomethacin enhanced attraction tumor-specific CTLs reducing regulatory attraction. Together, presented data broadens our understanding mechanisms DC-induced might lead improved vaccines.