Alp suppression of Lon: dependence on the slpA gene.

作者: J E Trempy , J E Kirby , S Gottesman

DOI: 10.1128/JB.176.7.2061-2067.1994

关键词: BiologyTranscriptional regulationMutantRegulator geneGeneTranscription (biology)PlasmidMolecular biologyProteaseOpen reading frame

摘要: We have previously found that plasmids carrying the Escherichia coli alp gene (now to be called alpA) suppress two phenotypes of a delta lon protease mutant, overproduction capsular polysaccharide and sensitivity UV light. Suppression these is most likely explained by increased degradation Lon substrates responsible for phenotypes. this suppressing activity Alp protease. The detected in cells after introduction alpA gene, which encodes an open reading frame 70 amino acids. Insertions abolish interrupt frame. used Tn10 lambda placMu mutagenesis identify chromosomal locus, slpA, required alpA+ suppression lon. This locus maps at 57 min, close location alpA. expression beta-galactosidase from lac transcriptional fusion slpA six- eightfold when present on multicopy plasmid. Therefore, AlpA acts as regulator gene(s); activation transcription necessary mutation. In accompanying paper (J. E. Kirby, J. Trempy, S. Gottesman, Bacteriol. 176:2068-2081, 1994), we show neither nor SlpA component itself but they are part regulatory cascade leads

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